ABSTRACT
<p><b>OBJECTIVE</b>To study the expressions of survivin, PTEN and their relationships with tissue grade and pathology stage in prostatic carcinoma (PCa).</p><p><b>METHODS</b>The immunohistological staining was used to evaluated the expressions of survivin protein and PTEN in 43 case of prostatic carcinoma (PCa) and 5 cases of benign prostatic hyperplasia (BPH).</p><p><b>RESULTS</b>The positive rate of survivin protein was 81.40%. Expression of survivin protein in 5 case of BPH was negative. The positive rate of PTEN was 30.23%, and the higher the grade and the clinical stage of tumors were, the lower the expression of PTEN was, PTEN of 5 case of BPH was positive.</p><p><b>CONCLUSION</b>The positively correlation was found between the abnormal expressions of survivin protein, PTEN and the biological behavior of prostatic carcinoma (PCa). Detection of survivin combined with PTEN is valuable for diagnosing PCa, and evaluating malignancy extent and prognosis.</p>
Subject(s)
Aged , Humans , Male , Middle Aged , Immunohistochemistry , Inhibitor of Apoptosis Proteins , Microtubule-Associated Proteins , Neoplasm Proteins , PTEN Phosphohydrolase , Prognosis , Prostatic Neoplasms , Metabolism , PathologyABSTRACT
<p><b>AIM</b>To observe the influence of polysaccharides of Angelica sinensis (ASP) on the immunologic function of rat Kupffer cells.</p><p><b>METHODS</b>Normal rat Kupffer cells were treated with ASP in vitro. Absorbance at 540 nm ( A540) of neutral red absorption and supernatant NO, TNF-alpha in the cells were measured to evaluate the immunologic function of Kupffer cells; LDH leakage was measured to estimate the severity of cellular damage; Rats were given ASP 0.025, 0.1, 0.25 and 1.0 g x kg(-1) ig (qd x 7 d) in vivo. The above indices and ACP of Kupffer cells were measured, sGST and sALT activity were detected as indices of hepatotoxicity.</p><p><b>RESULTS</b>ASP markedly enhanced the phagocytic activity, ACP and supernatant NO, TNF-alpha of Kupffer cells both in vitro and in vivo . The increase of sGST was observed after administration of ASP 1.0 g x kg(-1), but the LDH leakage of the hepatocytes was not increased in vitro.</p><p><b>CONCLUSION</b>ASP with suitable dose could activate the function of Kupffer cells. Slight liver injury was caused by ASP 1.0 g x kg(-1) in vivo, which was likely caused by factors, such as NO, TNF-alpha, indirectly.</p>
Subject(s)
Animals , Female , Male , Rats , Adjuvants, Immunologic , Pharmacology , Alanine Transaminase , Blood , Angelica sinensis , Chemistry , Cells, Cultured , Glutathione Transferase , Blood , Hepatocytes , Metabolism , Kupffer Cells , Allergy and Immunology , Metabolism , L-Lactate Dehydrogenase , Metabolism , Nitric Oxide , Metabolism , Phagocytosis , Plants, Medicinal , Chemistry , Polysaccharides , Pharmacology , Rats, Wistar , Tumor Necrosis Factor-alpha , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Angelica sinensis Polysaccharides (ASP) on the hepatic drug metabolism enzymes activities in normal mice and those prednisolone (PSL)-induced liver injury.</p><p><b>METHOD</b>The activities of phase II enzymes (GSH-related enzymes) and cytochrome P450 enzymes were measured by biochemical method.</p><p><b>RESULT</b>ASP increased the activities of glutathione S-transferase in liver microsomes and mitochondria. The cytochrome P450 content, NADPH-cytochrome c reductase, aminopyrine N-demethylase, and aniline hydroxylase activities in liver microsomes were also increased. PSL significantly increased serum ALT levels, and decreased the liver mitochondrial glutathione content. At the same time, other enzymes activities were all increased. When mice were treated with ASP 2.0 g.kg-1, the PSL-induced changes on cytochrome P450 enzymes, glutathione S-transferase, and GSH content were restored.</p><p><b>CONCLUSION</b>ASP can modulate the activities of drug metabolism enzymes.</p>